Content (click on the „G“ to view the glossary entry):

• Defintions and Separation Mechanisms,
• Stationary phases, (G), Mobile Phases (G) and Elution (G)
• Chromatographic parameters and van Deemter Equation (G)
• Normal Phase Chromatography (G)
• Reversed Phase Chromatography (G)
• Size Exclusion Chromatography (G)
• Ion Chromatography (G)
• Affinity Chromatography (G)
• Gas Chromatography (G)
• Supercritical Fluid Chromatography
• Hydrophobic Interaction Chromatography

Chromatographic Basics

Definitions and separation mechanisms:

Elution:

Reversed and Normal Phase Chromatography

Normal phase chromatography:

Reversed phase chromatography:

Normal and reversed stationary phases:

Two-dimensional Chromatography

Some further information about liquid chromatography provided by Agilent Technologies:

• LC Fundamentals Theory
• LC Fundamentals Hardware
• HPLC Column Selection Guide
• LC Handbook: Columns and Method Development
• Two-dimensional liquid chromatography

© 2015 Agilent Technologies Inc., all rights reserved, for teaching purpose only, used with permission

HPLC Application Notes provided by KNAUER:

• Guideline for method transfer from HPLC to UHPLC
• Ingredients in cough syrup
• Fast analysis of water pollutant pesticides
• HPLC Troubleshooting Guide
• Determination of coumarin in cinnamon products
• Determination of Naringine and Hesperidine in Fruit Juices

© 2011 KNAUER Wissenschaftliche Geräte GmbH, all rights reserved, used with permission

HPLC guide provided by Phenomenex LTD. (german):

• Liquid chromatography
• Stationary phase: Base materials
• Stationary phase: Ligands
• Chromatographic measurands
• Resolution

© Phenomenex Inc., all rights reserved, used with permission

Affinity Chromatography

Further information provided by GE Healthcare:

Affinity Chromatography – Handbook

© 2007 GE Healthcare Bio-Sciences AB, all rights reserved, used with permission

Ion Chromatography

Further information provided by Metrohm:

© 2018 Deutsche METROHM GmbH & Co. KG, all rights reserved, for teaching purpose only, used with permission

Further information provided by GE Healthcare:

Supercritical Fluid Chromatography

Further information published by Bieber and Letzel, provided by Agilent Technologies:

• SFC – Science Fiction Chromatography? (german)
• Why SFC? (german)
• Great potential of SFC (german)
• What column do I need?
• Effects on pressure
• SFC – Handbook

© 2015 Agilent Technologies Inc., all rights reservied, used with permission

Size Exclusion Chromatography

Further information provided by Agilent Technologies:

Introduction to SEC

© 2014 Agilent Technologies Inc., all rights reserved, used with permission

Hydrophobic Interaction Chromatography

Gas Chromatography

Further information provided by Agilent Technologies:

• GC Fundamentals – Theory
• GC Fundamentals – Hardware

© 2015 Agilent Technologies, all rights reserved, for teaching purpose only, used with permission

Hydrophilic Interaction Chromatography

Solutions of Greco and Letzel provided by Separation Science:

• Do you HILIC?
• The Basics of HILIC
• Stationary Phases
• Mobile Phase Solvents
• Mobile Phase Salt Content
• Mobile Phase pH
• Column Temperature
• Column Equilibration
• Sample Solvent
• HILIC and MS
• RPLC and HILIC coupling