This chapter includes slides about (click on the „G“ to view the glossary entry):

• Basic knowledge required for the understanding of bioanalytical methods (detergents (G) and the formation of detergent micelles & critical micellar concentration (G) (CMC), isoelectric point)
• Electrophoresis (Northern Blot (G)/ Southern Blot (G)/ Western Blot (G), SDS Page (G) and Native Page (G), Isoelectric focussing (G), 2D Electrophoresis (G))
• Immunoassays like ELISA (=enzyme-linked immunosorbent assay), FACS (=fluorescence activated cell sorting), FRET (=fluorescence resonance energy transfer), Dot Blot, etc.
• Videos about electrophoretic methods and immunoassays

Need some refreshment? No problem! Watch these videos and get your basic knowledge straight in no time.

• DNA replication, RNA transcription, translation
• Post-translational modifications
• Introduction to proteins
• Introduction to antibodies, function of antibodies, monoclonal antibodies

 

Got it? All right, let’s get started:

Electrophoresis:

Imunoassays:

 

Watch some more videos to go into more detail:

Western blotting provided by GE Healthcare Life Sciences:

ELISA provided by openmichigan:

Dot blot provided by Abnova: