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1. Sort the stationary phases by hydrophobicity (1 = highest hydrophobicity):
2. Match the numbers in the boxes with the words below.
a) biological interaction, b) hydrophobicity, c) size, d) charge
1a, 2b, 3d, 4c
3. Name the stationary phases shown below.
4. Label the HPLC parts.
5. Match the numbers in the boxes with the words below.
a) inhomogeneous packing material, b) movement alongside the column, c) small stationary phase particles, d) particles with narrow size distribution, e) high mobile flow rates, f) short and narrow tubings, g) entering of analyte into pores, h) low mobile flow rates, i) increased temperature
1a, 2cd, 3b, 4ef, 5g, 6hi
6. Find the correct names for the different terms of the Van Deemter equation.
7. Match the numbers in the brackets with the words below.
In reversed phase chromatography the mobile phase is (1) the stationary phase. (2) in reversed phase chromatography usually consist of water or (3) and an (4). (5) is based on the hydrophobicity of an analyte molecule, which can be expressed as (6). The retention time is (amongst others) influenced by (7) strength of the organic modifier and (8).
a) buffer, b) the retention time, c) mobile phase, d) organic modifier, e) logP, f) the temperature, g) more polar than, h) the solvent
1g, 2c, 3a, 4d, 5b, 6e, 7h, 8f
7. Rank the following compounds according to their adsorption ability in a decreasing order.
Amides, saturated hyrocabons, olefins, aldehydes, aromatics, nitro compounds, carboxylic acids, ketones, alcohols, amines, esters
[carboxylic acids] > [amides] > [amines] > [alcohols] > [ketones] > [aldehydes] > [esters] > [nitro compounds] > [aromatics] > [olefins] > [saturated hyrocabons]